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De novogenome set up and succession analyses

5). Duplicate sequences were got rid of towards the treat_duplicate program (CLC-bio) making use of the standard alternatives. Immediately following filter, genome libraries having inserts off 500 bp, 3 kb, and you will ten kb was in fact built with the AllPaths-LG (type 42411, ) formula which have standard details. The fresh new A beneficial. cerana genome succession can be acquired on the NCBI with project accession PRJNA235974. Repeat factors in the A beneficial. cerana genome were recognized using RepeatModeler (adaptation step one.0.eight, ) which have default choice. Subsequently, RepeatMasker (adaptation 4.03, ) was applied to help you display screen DNA sequences facing RepBase (change 20130422, ), brand new repeat database, and you may cover up most of the regions one coordinated recognized repetitive elementsparison away from fresh mitochondrial DNA to had written mitochondrial DNA (NCBI accession GQ162109) is actually performed using the CGView Server towards the standard choice . Continue reading